phase
1
Contact Us
Please contact us first.
We will be happy to suggest the best analysis method.
phase
2
Sampling and DNA extraction
Develop optimal sampling strategies based on research objectives and target environment.
We design appropriate collection methods for environmental samples and extract high quality DNA.
We use methods customized for each case to minimize contamination and preserve microbial diversity.
Depending on the sequencing method of choice (16S rRNA full-length amplicon, shotgun, or both), we apply the appropriate DNA extraction and purification protocols.
Expected duration: 1 to 2 weeks
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Detailed research objectives analysis and consultation
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Development of a customized sampling plan
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Collection of environmental samples
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Purpose-built high-quality DNA extraction and quality assessment
phase
3
Sequencing data acquisition
Highly accurate sequencing based on the chosen method is performed using the latest next-generation sequencers. Select or combine the methods best suited to your research objectives from the following options
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16S rRNA full-length amplicon sequencing: High resolution taxonomic diversity of bacteria
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Shotgun metagenomic sequencing (short reads): Analyze broad gene repertoire and functional potential
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Shotgun metagenome sequencing (long reads): Analysis of complex gene structures and repetitive sequences, high-precision genome reconstruction
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Hybrid approach: Comprehensive analysis combining short- and long-reads
For each approach, appropriate Sequencing depth and replicates are set to ensure reliable data generation.
Expected duration: 2-3 weeks
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Selection and optimization of sequencing methods to suit your research objectives
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Perform high-quality library preparation and sequencing
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Initial data quality assessment and QC report generation
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Reporting of data acquisition status and coordination of next steps
phase
4
Data analysis and microflora profiling
The acquired sequence data will be analyzed in detail using the latest bioinformatics tools. Depending on the sequencing method selected, the following analyses are performed
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16S rRNA full-length amplicon analysis:
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High-resolution taxonomic identification
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Phylogenetic analysis and calculation of diversity indices
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Shotgun metagenomic analysis: taxonomic and functional profiling
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Taxonomic and functional profiling
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Estimation of metabolic pathways and functional potential
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Discovery of novel genes and functions
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MAG (Metagenome-Assembled Genome) analysis
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Construction of high-quality genome bins
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Estimation of function and ecology of uncultured microorganisms
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In addition, correlation analysis with environmental factors and identification of important microbial groups are performed using advanced statistical methods and advanced algorithms.
Expected duration: 3 to 4 weeks
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Optimal analysis pipeline for selected methods
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Highly accurate taxonomic and functional profiling
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Statistical analysis to detect significant correlations and differences
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MAG construction and functional estimation of unknown microorganisms (if applicable)
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Integrated analysis with environmental factors
phase
5
Result interpretation and report writing
Based on the results of the analysis, we provide detailed interpretations of the structure, function, and ecological significance of the microbial community. We provide insight into environmental characteristics and their relationship to experimental conditions to meet your research objectives.
We also prepare custom reports tailored to your research needs, providing multifaceted visualization of results and detailed data analysis.
We also provide recommendations on next-step research strategies and offer ongoing research support.
Expected duration: 2-3 weeks
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Comprehensive interpretation of microbial community structure and function
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Correlation analysis with environmental factors and ecological considerations
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Detailed analysis of novel microbes and functions (if applicable)
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Advanced data visualization and comprehensive reporting
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Follow-up and recommendations for future research strategies
Bacterial flora of environmental microorganisms, etc. - Functional analysis process
Bio-DX
We use the latest sequencing technologies and advanced bioinformatics analysis to uncover the full extent of complex microbial communities, from 16S rRNA analysis to shotgun metagenomics and MAG analysis. We will support you to discover unknown microorganisms and functions in the environment.
16S rRNA full-length amplicon analysis (long reads)
35,000 yen 〜
(Excluding Tax, 1 sample)
High-resolution microflora profiling with advanced technology
Delivery time: 1 month
Shotgun metagenomic analysis (short reads)
56,000 yen 〜
(Excluding Tax, 1 sample)
High resolution taxonomic identification plus functional profiling
Delivery time: 1 month
