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phase

1

Consultation

Begin by reaching out to us. Our experts will propose the optimal analysis strategy for your unique requirements.

phase

2

Experimental Design and Sample Preparation

  • Analyze your R&D objectives in detail

  • Develop a customized experimental design

  • Create an optimal sampling plan

  • Extract high-quality RNA and conduct comprehensive quality assessment

Estimated duration: 1-2 weeks

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phase

3

Advanced Transcriptome Sequencing

  • Perform high-depth, high-accuracy sequencing using advanced high-throughput sequencing platforms

  • Strategically select the appropriate sequencing method (RNA-seq/Iso-seq) based on project goals

  • Optimize sequencing depth and replication for robust comparative characterization

  • Utilize advanced technologies:

    • Illumina RNA-seq for comprehensive gene expression quantification

    • PacBio Iso-seq for full-length transcript identification and isoform analysis

  • Conduct initial data quality assessment and generate QC reports

Estimated duration: 1-2 weeks

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phase

4

Data analysis In-depth Data Analysis and Gene Expression Profilingand gene expression profiling

  • Execute high-precision transcriptome analysis using validated bioinformatics tools

  • Identify differentially expressed genes with high sensitivity

  • Implement robust statistical approaches and algorithms for identification of differentially expressed genes and potential biomarkers

  • Key processes include:

    • Optimized analysis pipeline establishment

    • High-accuracy expression quantification and normalization

    • Differential expression analysis

    • Isoform analysis and alternative splicing detection

    • Functional enrichment analysis and biological interpretation

Estimated duration: 1-2 weeks

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phase

5

Expert Result Interpretation and Customized Reporting

  • Perform detailed interpretation of biological significance and potential applications of identified genes and isoforms

  • Develop tailored reports that address your specific research questions and business needs

  • Provide expert insights based on the latest scientific findings

  • Offer specialized analyses such as:

    • Pathway analysis and biological network mapping

    • Advanced data visualization

    • Strategic recommendations for future research directions

Delivery Time : 1-2 weeks

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High-Throughput Transcriptome Analysis for Industrially Useful Organisms

Bio-DX

At PtBio, we enable comprehensive transcriptome analysis for industrially useful organisms using RNA sequencing technologies, including RNA-seq and Iso-seq.

 

To meet your specific research needs, we offer tailored sequencing approaches, optimized for gene expression quantification, full-length transcript identification, and isoform characterization.

High-Precision RNA-seq Data Acquisition

35,000 yen 〜

(Excluding Tax, 1 Sample)

  • 3Gb (20 million paired-end reads) of high-quality RNA-seq data

  • Utilizing advanced sequencing technology for superior accuracy and throughput

Delivery: From 1 month

Comprehensive Candidate Gene and Marker Discovery for Genome Breeding

80,000 yen 〜

(Excluding Tax)

  • Identification, functional analysis, and data visualization of differentially expressed genes

  • Tailored strategies for genome breeding applications

Delivery: From 1 month

Service Plans

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